Unraveling the Structure of a Monoclonal Antibody
Join us for a webinar on Jul 27, 2016 at 1:00 PM EDT.
Barry E. Boyes, Ph.D.
Director of Bioscience Products
Advanced Materials Technology, Inc.
In this seminar, the focus will be on the HPLC / UHPLC characterization of biomolecules including: proteins, peptides, and glycosylation variants from a monoclonal antibody. A full review of a top-down and bottom-up approach to the analysis of trastuzumab will be explored.
This presentation will demonstrate how ultrahigh resolution LC-MS analysis of complex biomolecule mixtures can be achieved using conditions that permit excellent separations and analysis, including the use of new fused-core HPLC and UHPLC columns specifically designed for protein analysis. In addition, the consideration of novel mobile phase selection and conditions that favor high performance mass spectral analyses will also be discussed.
Specifically, we will discuss:
• Top-down and bottom-up reversed-phase LC–MS separations of trastuzumab
• The use of new fused-core HPLC and UHPLC columns designed for bio separations.
• Novel mobile phase selection for LC–MS analysis of monoclonal antibodies
• Separations for the analysis of glycosylation variants of monoclonal antibodies
Key Learning Objectives:
• How to characterize a monoclonal antibody with reversed-phase LC–MS and LC-UV separations
• How to utilize novel mobile phase conditions for improved mass spectral analysis of bio-separations
• Learn an optimized workflow for sample work-up and separation of glycosylation variants of monoclonal antibodies
Who Should Attend:
• Method developers for UHPLC and HPLC methods in biotech, biopharma, core laboratories and university research laboratories
• Method developers analyzing large biomolecules
• Method developers analyzing glycans or glycosylation variants of monoclonal antibodies
After registering, you will receive a confirmation email containing information about joining the webinar.