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A Step-by-Step Approach for Method Development to Generate a Successful HILIC Separation


Thu, Jun 15, 2017 12:00 AM - 1:00 AM CST


Alan P McKeown

Alan P McKeown BSc, PhD, CChem, FRSC
Business Development Director
Advanced Chromatography Technologies Ltd


Hydrophilic interaction liquid chromatography (HILIC) separations are gaining increased prominence in many application areas. The ability to retain polar neutral and ionizable polar species makes HILIC particularly popular for metabolomics and drug development studies. However, method development experience for HILIC is relatively scarce (or limited) in comparison to reversed-phase liquid chromatography (RPLC) in many laboratories. This seminar is designed to help the new HILIC chromatographer avoid the typical pitfalls that HILIC presents by establishing the critical parameters involved in HILIC separations. It will also demonstrate the relevance of a generally applicable selectivity-based HILIC method development platform to develop a HILIC related substances–purity method.


Yi He, Ph.D.
Professor of Chemistry Department of Sciences
John Jay College of Criminal Justice
The City University of New York