Please register for

How Much Separation for LC-MS/MS Quantitative Bioanalysis?


Wed, Oct 4, 2017 12:00 PM - 1:00 PM EDT


Aimin TanAimin Tan, Ph.D.
Director of Bioanalytical Laboratory
Nucro-Technics (Scarborough, Ontario, Canada)


LC–MS/MS has been the dominant analytical technology for quantitative bioanalysis for more than two decades. Despite this, a very fundamental question like how much separation is required for LC–MS/MS quantitative bioanalysis has not been adequately addressed. Some think that no or only very limited separation is necessary thanks to the unparalleled selectivity offered by tandem mass spectrometry. Others think that the more separation, the better, because of the potential detrimental impact of matrix effect. Still others just use a rule-of-thumb approach by keeping the adjusted retention/capacity factor always between 2 and 5. The purpose of this presentation is to address this fundamental question through rational thinking together with various real case examples.


Key Learning Objectives:

  • How to determine adequate separation to maximize method performance (accuracy, robustness, throughput, and cost-effectiveness)
  • What are the potential issues with quantitative bioanalysis?
  • Why can uHPLC or UPLC be a double-edged sword for quantitative Bioanalysis?


Who Should Attend:

  • Lab professionals using LC–MS for quantitation in Pharmaceutical companies, Biotech, Contract Research Organizations, and food-, clinical- and environment-testing laboratories
  • Scientists and sales persons involved in the development, application, and sales of chromatographic products and consumables
  • Professors and graduate students in the field of LC–MS quantitation


About Bonna-Agela:

Bonna-Agela Technologies is an innovative global supplier of chromatography separation and purification solutions, as well as complete sample preparation solution for biopharmaceutical, environmental and food industries. Our products and services include: HPLC and Flash chromatography purification, solid phase extraction cartridges and automatic workstation, and services from sub-gram to kilogram customized purification.