When: Wed, Jan 31, 2024 9:00 AM - 10:00 AM PST

Registration: https://register.gotowebinar.com/register/3074932038250028630

 

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Event Overview:

Mass spectrometry-based proteomics faces fundamental challenges in converting various types of biospecimens into LCMS-friendly samples. Currently methods often have high technical and economic barriers. EmporeTM membranes have been widely utilized by the proteomics community for rapid and convenient sample processing in the form of stop-and-go-extraction tips (StageTips). In this webinar, we will discuss recent developments of novel Empore membranes and their application in analyzing a variety of biological samples (e.g., cells, tissues, body fluids, and protein complexes) in an efficient, effective, and economical way, which we call E3technology. We will benchmark the performance of the new technologies against several established ones such as FASP, SPEED, SP4, and STrap, and demonstrate that E3technology provides equivalent or better performance in terms of proteome-wide identification and quantitation. Our new method based on enhanced E3technology, E4technology, also opens new avenues for low-input or low-cell proteomics analysis. We will further demonstrate their practical applicability by studying RNA-binding proteins derived from affinity purification and profiling the intact bacterial cell proteome. Overall, our data suggest that E3/E4 technologies are highly reliable, widely applicable, easily scalable, affordable, and feasible for non-expert proteomics laboratories. They represent a breakthrough innovation in biomedical science, and we anticipate their widespread adoption by the proteomics community.

 Key Learning Objectives:

  • Learn how the novel E3technology could be used universally to process your protein samples for mass spec analysis.
  • Learn how the novel E4 technology could process low-input cells, or even single cells for proteomics analysis.
  • Learn how to process pulldown or purified samples for mass spec analysis with minimal loss and high sensitivity.
  • Learn how an on-filter in-cell (OFIC) digestion method could revolutionize low-cell proteomics with easily accessible device.

 Who Should Attend:

 Scientists doing basic proteome analysis, biomarker discovery, or clinical proteomics.

  • Method developers for LC-MS/MS sample preparation in pharmaceutical, chemical, clinical, tox, forensic, environmental, agrichemical, contract research organization, universities, and governmental laboratories.
  • Graduate students, lab managers, directors and decision-makers.

Speaker’s long bio:

Dr. Yanbao Yu is currently the Director of Proteomics in the Department of Chemistry and Biochemistry at the University of Delaware (UD). He obtained his PhD in Chemical Biology from Fudan University in 2009 where he worked on blood platelet proteomics and innate immunity. He conducted post-doctoral research in UNC Chapel Hill, and later in the J. Craig Venter Institute (JCVI), where he focused on infectious disease, metaproteomics, and mass spectrometry-based method development. He was an Assistant Professor in JCVI prior to join UD in May 2021. Dr. Yu has keen interests in proteomics technology development and applications. He has published over 70 peer reviewed articles and invited book chapters, and has one pending patent.