Speaker's Title: Peter Pellegrinelli, M.S., Applications Specialist, Advanced Materials Technology, Inc. Event
Registration Link: https://register.gotowebinar.com/register/5966761965788878943
Overview: Join us to learn about a new phase chemistry from Advanced Materials Technology to improve LC and LCMS separations of basic compounds. With the release of the new HALO® Positive Charged Surface (PCS) stationary phase, chromatographers have another tool for better peak shapes and loading capacity of bases in addition to realizing the benefits of Fused-Core® particle technology for high speed, high efficiency separations. These new columns are designed for both small molecule and peptide analysis. Several applications will be shown to demonstrate:
• Improvement of peak shape for basic compounds using MS friendly mobile phases for both small molecule and peptide analysis
• Loading capacity improvement gains
• An HPLC method development approach for basic compounds with an alternative selectivity
Key Learning Objectives:
• How a positively charged surface column chemistry benefits LC & LCMS separations
• The advantages of a positively charged surface column with Fused-Core® technology
• Why using a low ionic strength mobile phase is desirable
Who Should Attend: Chromatographers and LC/MS method developers who are interested in improving their separations for basic compounds as well as investigating new column phase selectivities
About Sponsor: Advanced Materials Technology is an innovative HPLC column manufacturer focused on improving the presentation of the sample to the detector. Using novel Fused-Core® particle design, AMT has challenged conventional wisdom and engineered innovative solutions for the separations community. AMT continues to be a leader in novel superficially porous particle development enabling high efficiency and reliability for scientists running both small and large molecule separations in addition to delivering application specific solutions designed to meet industry challenges.
When: Wed, Jan 31, 2024 9:00 AM - 10:00 AM PST
Mass spectrometry-based proteomics faces fundamental challenges in converting various types of biospecimens into LCMS-friendly samples. Currently methods often have high technical and economic barriers. EmporeTM membranes have been widely utilized by the proteomics community for rapid and convenient sample processing in the form of stop-and-go-extraction tips (StageTips). In this webinar, we will discuss recent developments of novel Empore membranes and their application in analyzing a variety of biological samples (e.g., cells, tissues, body fluids, and protein complexes) in an efficient, effective, and economical way, which we call E3technology. We will benchmark the performance of the new technologies against several established ones such as FASP, SPEED, SP4, and STrap, and demonstrate that E3technology provides equivalent or better performance in terms of proteome-wide identification and quantitation. Our new method based on enhanced E3technology, E4technology, also opens new avenues for low-input or low-cell proteomics analysis. We will further demonstrate their practical applicability by studying RNA-binding proteins derived from affinity purification and profiling the intact bacterial cell proteome. Overall, our data suggest that E3/E4 technologies are highly reliable, widely applicable, easily scalable, affordable, and feasible for non-expert proteomics laboratories. They represent a breakthrough innovation in biomedical science, and we anticipate their widespread adoption by the proteomics community.
Key Learning Objectives:
- Learn how the novel E3technology could be used universally to process your protein samples for mass spec analysis.
- Learn how the novel E4 technology could process low-input cells, or even single cells for proteomics analysis.
- Learn how to process pulldown or purified samples for mass spec analysis with minimal loss and high sensitivity.
- Learn how an on-filter in-cell (OFIC) digestion method could revolutionize low-cell proteomics with easily accessible device.
Who Should Attend:
Scientists doing basic proteome analysis, biomarker discovery, or clinical proteomics.
- Method developers for LC-MS/MS sample preparation in pharmaceutical, chemical, clinical, tox, forensic, environmental, agrichemical, contract research organization, universities, and governmental laboratories.
- Graduate students, lab managers, directors and decision-makers.
Speaker’s long bio:
Dr. Yanbao Yu is currently the Director of Proteomics in the Department of Chemistry and Biochemistry at the University of Delaware (UD). He obtained his PhD in Chemical Biology from Fudan University in 2009 where he worked on blood platelet proteomics and innate immunity. He conducted post-doctoral research in UNC Chapel Hill, and later in the J. Craig Venter Institute (JCVI), where he focused on infectious disease, metaproteomics, and mass spectrometry-based method development. He was an Assistant Professor in JCVI prior to join UD in May 2021. Dr. Yu has keen interests in proteomics technology development and applications. He has published over 70 peer reviewed articles and invited book chapters, and has one pending patent.
It is with heavy hearts and profound respect that we announce this memorial symposium to honor the life and legacy of the esteemed Dr. Andrew J. Alpert. Dr. Alpert is a brilliant scientist, a dedicated mentor, a devoted collaborator, and a legend in the field of chromatography. Dr. Alpert's significant contributions left an indelible mark on the world of chromatography! Andy touched the lives of so many, he was always willing to stop what he was doing and help others. Dr. Alpert was also a talented artist, linguist (four languages), musician (pianist), sportsman (pole vaulter), chef, naturalist, and above all – a beloved husband, father, brother, and grandfather to five grandchildren.
The symposium will feature distinguished speakers, colleagues, and friends together with his family members who will reflect upon his groundbreaking research, his pioneering ideas, and the lasting impact he had on the liquid chromatography and mass spectrometry/proteomics. This event will provide an opportunity for family members, friends, colleagues, mentees, and admirers of Dr. Alpert to come together and share their memories, stories, and research. We aim to celebrate his legacy by highlighting the extraordinary contributions he made to the field of chromatography and mass spectrometry-based proteomics as well as the personal connections he forged throughout his life.
In addition to the speakers, there will be opportunities for attendees to join the virtual reception, engage in networking sessions, and pay their respects through heartfelt tributes. The symposium aims to foster an atmosphere of intellectual exchange, inspiration, and remembrance, just as Dr. Alpert did throughout his distinguished career.
We encourage all those whose lives were touched by Dr. Alpert to join us in this meaningful event. Whether you were a colleague, a student, a collaborator, or an admirer from afar, your presence will help us create a fitting tribute to a remarkable individual who will forever remain in our hearts and minds.
Please stay tuned for further details regarding the agenda, speakers, and registration process. Let us come together to celebrate the life and legacy of Dr. Andrew J. Alpert!
With deepest respect,
Organizers (CACA, PolyLC, Inc, 仪器信息网, and Ge Research Group)
Mon, Jun 26, 2023 9:00 AM - 10:00 AM PDT
Speaker's Title: Catharine Layton, Principal Scientist, Scientific Operations, Waters Corporation
Event Overview: On December 1, 2022 a harmonized standard for General Chapter 621 Chromatography was released. This standard incorporates the USP with 2.2.46. Chromatographic Separation Techniques European Pharmacopoeia (EuPh) and 2.01 Liquid Chromatography Japanese Pharmacopeia (JP) texts. Harmonization of these regulatory guidance provides increased method flexibility through the employment of modernized of chromatographic tools without the need for full monograph re-validation.
The extent to which the various parameters of a compendial chromatographic test may be adjusted without fundamentally modifying the pharmacopeial analytical procedures is defined in U.S. Pharmacopeia (USP) General Chapter 621 Chromatography. In this presentation, we combine the gradient method adjustments described in this chapter to achieve both column and system modernization for the USP monograph separation of antiviral drug abacavir sulfate.
Key Learning Objectives:
• Learn how to modernize the HPLC column and reduce operation costs using the USP 621 allowable adjustments
• Learn how to modernize the LC platform and further reduce operation costs using the USP 621 allowable adjustments
• Leverage Empower CDS fields to apply the updated chromatography reporting calculations
Who Should Attend:
• Scientists and lab managers involved with compendial methods with a drive towards modernization, productivity and compliance.
Speaker: Yu Tian, PhD, Director, Omics Technology, Drug Metabolism and Pharmacokinetics, AbbVie Bioresearch Center
When:Time: June 15, 2022
Many fibrotic and inflammatory diseases such as idiopathic pulmonary fibrosis (IPF) and hidradenitis suppurativa (HS) are pathologically and spatially heterogeneous, posing challenges for traditional bulk analysis approaches that fail to capture spatial information. Laser capture microdissection (LCM) is a powerful technology for spatial omics analysis, which allows histopathology-guided sample area selection to achieve fine spatial resolution and enable specific and strong association of biology data with pathology results. However, it is still challenging to conduct downstream omics analysis due to the limited quantity of tissue material after high-resolution LCM area selection.
In this webinar, we will introduce an LCM proteomics workflow that has been established and optimized, including FFPE (formalin-fixed, paraffin-embedded) tissue sectioning, tissue harvesting by LCM, StageTip-assisted proteomics sample handling and preparation, LC/MS instrument settings, and proteomics database search strategy. The technical pilot study was conducted using human IPF lung and HS patient skin LCM samples with pathological features. Multiple differential expression analysis was performed between LCM regions, with a panel of extracellular matrix (ECM) related targets identified with a relationship to fibrosis mechanism.
Key Learning Objectives:
- Compare performance of state-of-art LCM sample preparation procedures
- Optimize LCM-based spatial proteomics workflow
- Know the promising applications of the developed spatial proteomics workflow on fibrosis-related disease understanding
Who Should Attend:
- Method developers for spatial proteomics in pharmaceutical, chemical, clinical, contract research organization, university, and governmental laboratories.
- Method developers analyzing quantity-limited samples
- Biologists for fibrosis-related disease investigation