Speaker: Stephanie A. Schuster, Senior Technical Support Scientist, Advanced Materials Technology, Inc.
When: Wed, Sep 22, 2021 9:00 AM - 10:00 AM PDT
About the Speaker: Dr. Schuster earned her B.A. at La Salle University, and her Ph.D. under the guidance of Prof. Joe P. Foley at Drexel University. After graduation, Stephanie spent nine months at Atlantic Diagnostic Laboratories, a combined clinical and forensic testing laboratory. She joined Advanced Materials Technology, Inc. (AMT) located in Wilmington, DE in June 2009 as a research scientist working with Dr. Jack Kirkland, providing contributions to the commercial development of products designed specifically for the separation of peptides and proteins. Most recently, as part of the Technical Support team at AMT, Stephanie has been providing customers support in optimizing and trouble-shooting their applications. Stephanie has several peer-reviewed articles and is a member of the Chromatography Forum of the Delaware Valley (CFDV) and the American Society of Mass Spectrometry (ASMS).
Abstract: Monoclonal antibodies (mAbs) continue to be among the top selling pharmaceuticals because they are such effective therapeutics. Not only are mAbs used for disease treatment, but they are also used for research and diagnostic purposes. Characterization of mAbs is a critical step in their development. Intact analysis may be completed by LC-UV or LC-MS methods using Fused-Core particle columns. Additionally, post-translational modifications (PTMs) of mAbs must be characterized and controlled. Many PTMs are polar by their nature, including glycosylation, deamidation, and oxidation. These types of polar modifications lend themselves to analysis via hydrophilic interaction liquid chromatography (HILIC) since they can be better resolved than with reversed-phase chromatography. Examples of glycosylation, deamidation, and oxidation of different mAbs will be demonstrated using LCMS under HILIC conditions.