Speaker: Peter Pellegrinelli, M.S. Application Specialist Advanced Materials Technology, Inc.
When: Nov 1st, 2023 9:00 AM - 10:00 AM PDT
Chromatographers continue to explore alternate avenues to improve their chromatography separations. These avenues can encompass different parameters including connection tubing, instrumentation configuration, column choice, amongst others. If the method requirements permit freedom for change, adjusting method parameters or exchanging the column are simple changes. It is well known that by switching from larger ID columns to a smaller ID can reduce overall solvent consumption and improve sensitivity. Previously, in order to improve the method sensitivity using a 2.1 mm ID, a specialized micro or nano flow LC system using capillary columns was used. However, this can be an expensive venture to undertake to improve one’s chromatography system performance from a routine operational and maintenance standpoint.
In this webinar a new 1.5mm ID column technology will be discussed. Skipping the expense of a specialized system and gain improvements in both sensitivity and reduced solvent consumption with a commercial UHPLC system will also be presented. This new column dimension shows an increase in sensitivity through both UV and MS applications. With greater response and higher ionization efficiencies problematic separations can be overcome. Advantages to the proteomic and peptide mapping applications will be shown along with some small molecule application examples.
Key Learning Objectives:
• How a new 1.5 mm ID column delivers an increase in sensitivity for UHPLC and LCMS separations
• Ability to implement and achieve green initiatives with new column technology
• Getting more performance from your UHPLC system
Who Should Attend:
• Chromatographers developing methods for large and small molecule separations
• UHPLC users interested in increasing sensitivity
• Researchers looking to reduce solvent consumption