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Mon, Jun 26, 2023 9:00 AM - 10:00 AM PDT

Registration: https://register.gotowebinar.com/register/6740620237533843291

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Speaker's Title: Catharine Layton, Principal Scientist, Scientific Operations, Waters Corporation

Event Overview: On December 1, 2022 a harmonized standard for General Chapter 621 Chromatography was released. This standard incorporates the USP with 2.2.46. Chromatographic Separation Techniques European Pharmacopoeia (EuPh) and 2.01 Liquid Chromatography Japanese Pharmacopeia (JP) texts. Harmonization of these regulatory guidance provides increased method flexibility through the employment of modernized of chromatographic tools without the need for full monograph re-validation.

The extent to which the various parameters of a compendial chromatographic test may be adjusted without fundamentally modifying the pharmacopeial analytical procedures is defined in U.S. Pharmacopeia (USP) General Chapter 621 Chromatography. In this presentation, we combine the gradient method adjustments described in this chapter to achieve both column and system modernization for the USP monograph separation of antiviral drug abacavir sulfate.

Key Learning Objectives:

• Learn how to modernize the HPLC column and reduce operation costs using the USP 621 allowable adjustments

• Learn how to modernize the LC platform and further reduce operation costs using the USP 621 allowable adjustments

• Leverage Empower CDS fields to apply the updated chromatography reporting calculations

Who Should Attend:

• Scientists and lab managers involved with compendial methods with a drive towards modernization, productivity and compliance.

Speaker:  Yu Tian, PhD, Director, Omics Technology, Drug Metabolism and Pharmacokinetics, AbbVie Bioresearch Center

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When:Time: June 15, 2022

Event Overview:

Many fibrotic and inflammatory diseases such as idiopathic pulmonary fibrosis (IPF) and hidradenitis suppurativa (HS) are pathologically and spatially heterogeneous, posing challenges for traditional bulk analysis approaches that fail to capture spatial information. Laser capture microdissection (LCM) is a powerful technology for spatial omics analysis, which allows histopathology-guided sample area selection to achieve fine spatial resolution and enable specific and strong association of biology data with pathology results. However, it is still challenging to conduct downstream omics analysis due to the limited quantity of tissue material after high-resolution LCM area selection.

In this webinar, we will introduce an LCM proteomics workflow that has been established and optimized, including FFPE (formalin-fixed, paraffin-embedded) tissue sectioning, tissue harvesting by LCM, StageTip-assisted proteomics sample handling and preparation, LC/MS instrument settings, and proteomics database search strategy. The technical pilot study was conducted using human IPF lung and HS patient skin LCM samples with pathological features. Multiple differential expression analysis was performed between LCM regions, with a panel of extracellular matrix (ECM) related targets identified with a relationship to fibrosis mechanism.

 

Key Learning Objectives:

  • Compare performance of state-of-art LCM sample preparation procedures
  • Optimize LCM-based spatial proteomics workflow
  • Know the promising applications of the developed spatial proteomics workflow on fibrosis-related disease understanding

Who Should Attend:

  • Method developers for spatial proteomics in pharmaceutical, chemical, clinical, contract research organization, university, and governmental laboratories.
  • Method developers analyzing quantity-limited samples
  • Biologists for fibrosis-related disease investigation

 

     Speaker: Peter Pellegrinelli, M.S. Application Specialist Advanced Materials Technology, Inc.
 
 
When: Wed, Aug 17, 2022 9:00 AM - 10:00 AM PDT
 
Chromatographers continue to explore alternate avenues to improve their chromatography separations. These avenues can encompass different parameters including connection tubing, instrumentation configuration, column choice, amongst others. If the method requirements permit freedom for change, adjusting method parameters or exchanging the column are simple changes. It is well known that by switching from larger ID columns to a smaller ID can reduce overall solvent consumption and improve sensitivity. Previously, in order to improve the method sensitivity using a 2.1 mm ID, a specialized micro or nano flow LC system using capillary columns was used. However, this can be an expensive venture to undertake to improve one’s chromatography system performance from a routine operational and maintenance standpoint.
 
In this webinar a new 1.5mm ID column technology will be discussed. Skipping the expense of a specialized system and gain improvements in both sensitivity and reduced solvent consumption with a commercial UHPLC system will also be presented. This new column dimension shows an increase in sensitivity through both UV and MS applications. With greater response and higher ionization efficiencies problematic separations can be overcome. Advantages to the proteomic and peptide mapping applications will be shown along with some small molecule application examples.
 
Key Learning Objectives:
• How a new 1.5 mm ID column delivers an increase in sensitivity for UHPLC and LCMS separations
• Ability to implement and achieve green initiatives with new column technology
• Getting more performance from your UHPLC system
 
Who Should Attend:
• Chromatographers developing methods for large and small molecule separations
• UHPLC users interested in increasing sensitivity
• Researchers looking to reduce solvent consumption

Speakers:
• Catharine Layton, Principal Scientist, Scientific Operations, Waters Corporation 
• Martin Gilar, Ph. D., Scientific Fellow, Separations R&D, Waters Corporation 
 
When: Wed, Jul 20, 2022 9:00 AM - 10:00 AM PDT
Event Overview: 
Non-specific binding of analytes to material surfaces is an inherent characteristic of conventional chromatographic columns and systems. This phenomenon leads to reduced sensitivity and sometimes poor peak shape of acidic molecules. In this webinar we will present a new, novel hybrid surface technology that provides a solution without the need for strong mobile phase additives, chelators, or lengthy passivation protocols. The chromatographic benefits provided when using columns and systems equipped with the MaxPeak™ High Performance Surfaces (HPS) technology will be demonstrated with antivirals, NSAIDs, cancer growth inhibitors, oligonucleotides, peptides, and glycans. 
 
Key Learning Objectives: 
• Understand how metal-analyte interactions can negatively impact chromatographic performance. 
• Learn how to mitigate metal-analyte interactions with the use of a novel surface technology. 
• Explore applications: antivirals, NSAIDs, cancer growth inhibitors, oligonucleotides, peptides and glycans. 
 
Who Should Attend: 
• Scientists who develop and/or manage UHPLC and HPLC methods in pharmaceutical laboratories. 
• Lab managers leading teams involved with the analysis of small and large molecules.
 

 
Speakers: Title: Peter Ratsep, Ph.D. (Technical Support Supervisor) and Sarah Braseth (Regional Manager), Shimadzu Scientific Instruments NJ office
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When: Wed, Oct 20, 2021 9:00 AM - 10:00 AM PDT
 
Event Overview: Many laboratories today are faced with the challenge of running multiple different analyses on the same sample. This challenge can present itself when there is an increase in sample complexity, additional sample regulations and requirements that must be met, when sampling is done at different time markers, when you are scouting for the right method settings, or if you are doing general API analysis to meet different USP requirements. The Shimadzu Nexera Dual Injection (U)HPLC system is equipped with two injection ports (independent flow paths) which allows two analyses to be run simultaneously on a single instrument, potentially decreasing your analysis time by 50%. This webinar will highlight specific applications of simultaneous analyses done on one sample with one (U)HPLC. In addition, this webinar will also explain how the two sets of data are then integrated into a single data file simplifying data management and ensuring data integrity.
 
Key learning Objectives:
• Understanding the advantages of a dual injection system
• Learn a novel approach to address efficiency when running a single sample under multiple different analyses conditions
• Maintaining data integrity and data tracking by combining data and method files from multiple runs
 
Who Should Attend:
• Method developers in pharmaceutical, foods and cosmetics, chemical, environmental, agrichemical, contract research organization, university and governmental laboratories.
• Scientists working in an HPLC laboratory looking to increase throughput and efficiency with their workflows
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